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KMID : 0848120130380030093
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International Journal of Oral Biology
2013 Volume.38 No. 3 p.93 ~ p.100
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The Role of HS-1200 Induced Autophagy in Oral Cancer Cells
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Park Bong-Soo
Jang Nam-Mi
Oh Sang-Hun
Kim In-Ryoung
Park Hae-Ryoun
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Abstract
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Bile acids and synthetic bile acid derivatives induce apoptosisin various kinds of cancer cells and thus have anticancerproperties. Recently, it has been suggested that autophagymay play an important role in cancer therapy. However,few data are available regarding the role of autophagy inoral cancers and there have been no reports of autophagic celldeath in OSCCs (oral squamous cell carcinoma cells) inducedby HS-1200, a synthetic bile acid derivative. We thusexamine whether HS-1200 modulates autophagy in OSCCs.Our findings indicate that HS-1200 has anticancer effects inOSCCs, and we observed in these cells that autophagic vacuoleswere visible by monodansylcadaverine (MDC)and acridineorange staining. When we analyzed HS-1200-treated OSCCcells for the presence of biochemical markers, we observedthat this treatment directly affects the conversion of LC-3II,degradation of p62/SQSTM1 and full-length beclin-1, cleavageof ATG5-12 and the activation of caspase. An autophagyinhibitor suppressed HS-1200-induced cell death in OSCCs,confirming that autophagy acts as a pro-death signal inthese cells. Furthermore, HS-1200 shows anticancer activityagainst OSCCs via both autophagy and apoptosis. Ourcurrent findings suggest that HS-1200 may potentially contributeto oral cancer treatment and thus provide useful informationfor the future development of a new therapeutic agent.
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KEYWORD
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HS-1200, autophagy, apoptosis, OSCC
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